Device

Part:BBa_K1548003

Designed by: Michael Jacus   Group: iGEM14_Pitt   (2014-10-07)


FixK2 Promoter-RBS-mRFP1-Hsp60 Promoter-RBS-YF1-RBS-FixJ

System for blue light-induced expression of mRFP1 in P. acnes using constitutive Hsp60 promoter from M. bovis (expected to work in P. acnes) to drive expression of blue light-regulated transcription factors YF1 and FixJ.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1913
    Illegal NgoMIV site found at 1985
    Illegal NgoMIV site found at 2075
    Illegal NgoMIV site found at 2093
    Illegal NgoMIV site found at 2605
    Illegal NgoMIV site found at 2898
    Illegal NgoMIV site found at 2992
    Illegal AgeI site found at 831
    Illegal AgeI site found at 943
    Illegal AgeI site found at 1185
    Illegal AgeI site found at 1627
    Illegal AgeI site found at 2773
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2662
    Illegal BsaI.rc site found at 1526


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Categories
Parameters
None