Composite

Part:BBa_K1547000

Designed by: victor yuan   Group: iGEM14_Concordia   (2014-10-08)

PSAD Promoter and Terminator, with GFP reporter gene

High constitutive PSAD promoter and terminator sequences flanking a GFP reporter gene for high expression and easy characterization. This cassette was transformed into chlamydomonas reinhardtii (iGEM Concordia 2014). Genomic DNA extraction was performed and a PCR confirmed the presence of the construct had integrated into the chlamydomonas genome. Further characterization of this construct can include fluorescent microscopy to see GFP expression, and also quantification using FLOW cytometry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1570
    Illegal AgeI site found at 916
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1679


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