Regulatory

Part:BBa_K1545002

Designed by: Mike Zhu   Group: iGEM14_Duke   (2014-10-09)

12xGFP1 decoys

Array of 12 binding sites for gRNAs matching CCATCTAATTCAACAAGAATTGG + 7 bp spacer.

Use this as a decoy to change degree of repression by dCas9 of GFP (BBa_K608012). Bacteria harboring dCas9 targeting this site have less repression of GFP in proportion to the copies of decoy present as shown below:

BiggerDecoyReflow.jpg

Addition of pSB1A2-K154501 to strains containing pSB4K5-K608012 and pdCas9 with crRNA targeting GFP1 sequence 9.5-fold relative to pSB1A2 with no decoys.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None