Primer
HE Promote

Part:BBa_K1539055

Designed by: Coleen Tran   Group: iGEM14_GeorgiaTech   (2014-10-10)


HE Promoter Primer

This primer can be used to insert a high efficiency promoter to translate E. coli. genes. Must be used after insertion of RBS primer. Use Georgia Tech iGEM RBS primers (BBa_K1539021 or BBa_K1539034) or an RBS primer with a similar design.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 11
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 24
    Illegal NheI site found at 47
    Illegal NotI site found at 2
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 11
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None