Primer
HE RBS

Part:BBa_K1539021

Designed by: Coleen Tran   Group: iGEM14_GeorgiaTech   (2014-10-10)


HE RBS Primer

This primer can be used to insert a high efficiency RBS to transcribe coding sequences starting with ATG in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3
    Illegal NotI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3
    Illegal XbaI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None