Composite

Part:BBa_K1526004

Designed by: Cau� Westmann   Group: iGEM14_York   (2014-10-07)

pYoda + SpPCS

pYoda (cadmium inducible promoter) regulating the expression of the downstream gene Phytochechalatin Synthase from S. pombe

This Biobrick was created as both an intermediate for our final construct and as testable system for the functionality of our Phytochelatin Synthase gene under the control of the Cadmium inducible promoter PyodA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 428
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None