Part:BBa_K1526001

Gsh1*: Mutated Glutamate-Cysteine Ligase(Gsh1) from Saccharomyces cerevisiae

Glutamate-Cysteine Ligase(previously known as Gamma glutamylcysteine synthetase) catalyzes the first step in glutathione (GSH) biosynthesis;

L-glutamate + L-cysteine + ATP -> gamma-glutamyl cysteine + ADP + Pi

Expression is induced by oxidants, cadmium, and mercury. Protein abundance increases in response to DNA replication stress. (http://biocyc.org/YEAST/NEW-IMAGE?type=GENE-IN-MAP-IN-PWY&object=YJL101C)

Gsh1* can be used to overproduce cysteine to make gamma-glutamyl cysteine, which is the monomer that forms phytochelatins (n=10-20). In order to do this, glutamate-cysteine ligase is needed to catalyse the reaction. We can overexpress GSH1* using the pYodA(BBa K896008) promoter.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 234
  
• 1000
  COMPATIBLE WITH RFC[1000]