Part:BBa_K1523025

ChrR coding sequence

It's a device which can reduce Cr(VI) from P. putida.

It is called yieF gene in E.Coli K12. It can express the flavoprotein as a kind of chromate reductase.

Involved in the protection against chromate toxicity. Catalyzes the transfer of three electrons to Cr6+ producing Cr3+ and one electron to molecular oxygen without producing the toxic Cr5+ species and only producing a minimal amount of reactive oxygen species (ROS). It can also reduce quinones, potassium ferricyanide, 2,6-dichloroindophenol, V5+, Mo6+, methylene blue and cytochrome c. The quinone reductase activity may protect against oxidative stress by preventing redox cycling of quinones which would otherwise generate ROS and by maintaining a pool of reduced quinone in the cell that is able to quench ROS directly. It is able to use both NAD or NADP equally well.

Catalytic activity

NAD(P)H + a quinone = NAD(P)+ + a hydroquinone.

2 NAD(P)H + Cr6+ + O2 = 2 NAD(P)+ + Cr3+ + H2O2.

Structure

This picture is from RCSB

Reference Uniprot : http://www.uniprot.org/uniprot/H0QEW2