Coding

Part:BBa_K1523021

Designed by: Haotian Wang   Group: iGEM14_LZU-China   (2014-10-03)

nahR coding sequence,encoding a LysR-type transcriptional regulator

nahR, encoding a LysR-type transcriptional regulator, is highly conserved among naphthalene-degrading bacteria isolated from a coal tar waste-contaminated site and in extracted community DNA. It contains a kind of HTH LysR DNA-binding domain.

NAH7 plasmid specify catabolism of naphthalene and salicylate under positive regulation by gene nahR. Clones from the nahR gene region were expressed in mexicells by You in 1988. Plasmid pY1923, carrying the 1.75-kb PstI-HindIII fragment, expressed a protein of Mr ca. 35,000 which bound to the upstream region of gene nahR.

Operons repressed by nahR can be regulated positively by nahR when salicylate appears.


Vector Nah.jpg

The Vector NAH7. Both nah operon and sal operon are regulated by nahR.


Reference


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 57
    Illegal PstI site found at 250
    Illegal PstI site found at 469
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 57
    Illegal PstI site found at 250
    Illegal PstI site found at 469
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 323
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 57
    Illegal PstI site found at 250
    Illegal PstI site found at 469
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 57
    Illegal PstI site found at 250
    Illegal PstI site found at 469
    Illegal NgoMIV site found at 491
    Illegal NgoMIV site found at 646
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/anahR coding sequence,encoding a LysR-type transcriptional regulator