Intermediate

Part:BBa_K1521002

Designed by: Danilo Keiji Zampronio   Group: iGEM14_USP-UNESP-UFSCar-BRA   (2014-10-17)


LasR functional construction + GFP reporter

This was a intermidiate caracterization construction designed to evaluate the LasR-QteE interaction. In this construction there is no QteE being produced, while there is constitutive LasR expression. Having said that, what is expected to be seen here is a GFP fluorescence due to the activation of the PlasR promoter by LasR protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2174
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2055
    Illegal BglII site found at 4576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 585
    Illegal AgeI site found at 782
    Illegal AgeI site found at 1617
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2946


[edit]
Categories
Parameters
None