Translational_Unit

Part:BBa_K1521001

Designed by: Danilo Keiji Zampronio   Group: iGEM14_Brasil-SP   (2014-10-01)


RBS SpoVG (K143021) + lasR

This is an assembly os the biobricks SpoVG (K143021) for Bacillus subtilis and the LasR protein cds. Note that the lasR sequence was obtained through PCR of the part C0079 and that its LVA tag was also removed.

Although the RBS was designed for B. subtilis also works on E. coli.

This protein requires an HSL (homoserine lactone) produced by the gene lasI (C0078) to work appropriately. If you want the lasI is already available with RBS, cds, terminator and a promoter responsive to LasR (K081001).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 340
    Illegal AgeI site found at 537
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None