Translational_Unit

Part:BBa_K1521000

Designed by: Danilo Keiji Zampronio   Group: iGEM14_Brasil-SP   (2014-10-01)


Quorum threshold expression element (qteE) from Pseudomonas aeruginosa + SpoVg RBS

The qteE is the main quorum sensing regulator in Pseudomonas Aeruginosa. In this bacteria it is expressed as a constitutive protein that interacts with other quorum sensing proteins, such as LasR, inhibiting them from their regulation role (inducer, inhibitor and other functions). Assemblying this protein with specific promoters we are able to control the quorum sensing threshold, which is and interesting feature for pathways activation control. Moreover, if we assemble the qteE with an inducible promoter like Plac, it is possible to induce it with different IPTG concentrations, creating distinct threshold for protein production screening and even quantitative purposes.

This conding sequence comes with the SpoVg RBS (BBa_K143021) for usage in Bacillus subtilis. We tested it in E. coli and it worked as well.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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