Composite

Part:BBa_K1520520

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-26)

PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter

PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter It is part of our test of suicide circuit by gold ions.

Suicidewithoutgold.jpg

this is part of our part BBa_K1520509 [1] and the picture is also part of BBa_K1520509, since BBa_K1520509 is based on this one and our result shows it works, this one would definitely work.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 548
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
Parameters
biology
controlaTc, tetracyline
directionForward
functionRep
ligandstetracycline
negative_regulators1
o_h
o_l
positive_regulators
proteinTetR
swissproP04483
tagLVA