Composite

Part:BBa_K1520510

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-23)


PgolTS-golS-PgolB-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter

PgolTS-golS-PgolB-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter It is a test of our positive feedback circuit for gold ions.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1574
    Illegal NheI site found at 1597
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1416
    Illegal BglII site found at 3418
    Illegal BglII site found at 4088
    Illegal XhoI site found at 3390
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 548
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2552


[edit]
Categories
//function/biosynthesis/ahl
//function/cellsignalling
Parameters
biology
controlluxR, HSL
directionForward
ec_numnone
functionMixed
keggnone
ligandsHSL
n/aconstitutive promoter family member
negative_regulators
o_h
o_l
positive_regulators1
proteinluxI-LVA
swissproP12747
tagLVA
uniprotP48445