Part:BBa_K1520509

PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter

PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter It is a test of our suicide circuit for gold ions. It can express rfp when both gold ions and IPTG are not exist. Here rfp represent lysozyme, since the plasmid with lysozyme is hard to reconstruct. When the bacteria turns red, it means they commit suicide and the following pictures shows that the system work.

From left to right: 1.add gold 2.without gold 3.add IPTG 4.without IPTG

The fluorescence intensity of each treatment

From left to right, Marker(1Kb),

PgolTS-golB-PgolB-tetR-Ter-Ptet-rbs-rfp-Ter,

PgolTS-golB-PgolB-tetR-Ter-Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter.

The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 3611
  Illegal NheI site found at 3634
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 4812
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 548
  
• 1000
  COMPATIBLE WITH RFC[1000]