Composite

Part:BBa_K1520508

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-23)

PgolTS-golS-PgolB-rbs-luxI-Ter

PgolTS-golS-PgolB-rbs-luxI-Ter If there are gold ions, the luxI will be produced, which will result in the producing of AHL.

K1520508.jpg

From left to right, Marker(DL10000), PgolTS-golS-PgolB-rbs-tetR-Ter

The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1416
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 548
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/biosynthesis/ahl
//function/cellsignalling
Parameters
directionForward
ec_numnone
keggnone
proteinluxI-LVA
swissproP12747
tagLVA
uniprotP48445