RNA

Part:BBa_K152011

Designed by: David Johnston Monje   Group: iGEM08_Guelph   (2008-10-30)


GFP RNAi construct

This part needs a strong promoter inserted to drive its expression. It could then be transfered into another plasmid by restriction with EcoRI and PstI. We intend to electroporate it into a corn plant endophyte Klebsiella pneumonii 342, and inject this into GFP Arabidopsis for testing of the bacteria's ability to silence plant genes using RNAi plasmids.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 898
    Illegal XbaI site found at 907
    Illegal SpeI site found at 1038
    Illegal PstI site found at 1102
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 898
    Illegal SpeI site found at 1038
    Illegal PstI site found at 1102
    Illegal NotI site found at 1046
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 898
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 898
    Illegal XbaI site found at 907
    Illegal SpeI site found at 1038
    Illegal PstI site found at 1102
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 898
    Illegal XbaI site found at 907
    Illegal SpeI site found at 1038
    Illegal PstI site found at 1102
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 996
    Illegal BsaI.rc site found at 663
    Illegal BsaI.rc site found at 1780


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