RNA

Part:BBa_K152010

Designed by: David Johnston Monje   Group: iGEM08_Guelph   (2008-10-30)


TB1 RNAi construct

This is meant to be cut out using NdeI and PstI for ligation downstream of the strong promoter pDSK-GFPuv, followed by electroporation into the corn endophyte Klebsiella pneumonii 342. The expected goal is to see transcriptional knockdown of the gene in the plant when these bacteria are injected into it.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 254
    Illegal XbaI site found at 263
    Illegal SpeI site found at 394
    Illegal PstI site found at 458
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 254
    Illegal SpeI site found at 394
    Illegal PstI site found at 458
    Illegal NotI site found at 402
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 254
    Illegal BamHI site found at 112
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 254
    Illegal XbaI site found at 263
    Illegal SpeI site found at 394
    Illegal PstI site found at 458
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 254
    Illegal XbaI site found at 263
    Illegal SpeI site found at 394
    Illegal PstI site found at 458
    Illegal NgoMIV site found at 66
    Illegal NgoMIV site found at 72
    Illegal NgoMIV site found at 181
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 352


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