Composite

Part:BBa_K152004

Designed by: David Johnston Monje   Group: iGEM08_Guelph   (2008-10-30)


Promoterless Synthetic Operon of CrtE,B,I,Y

This operon provides the full set of bacterial open reading frames necessary for beta carotene production. crtE,B,I,Y coding regions from Erwinia uredovora each with a rbs preceeding the open reading frame. This synthetic operon could be made by ligating together the starter biobrick containing CrtE cut with SpeI and PstI, then ligating in the next XbaI/PstI cut fragment. There is no promoter or trancription termination signal on this construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1941
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1478
    Illegal NgoMIV site found at 1608
    Illegal AgeI site found at 732
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None