Composite
Part:BBa_K1520015
Designed by: yue wu Group: iGEM14_Nanjing-China (2014-09-22)
Plac-rbs-tetR-Ter
Plac-rbs-tetR-Ter. It produces tetR when there is enough IPTG or less lacI.
From left to right, Marker（DL2000）, Plac, Plac-rbs-tetR, Plac-rbs-tetR-T.
The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
//function/regulation/transcriptional
| biology | |
| control | IPTG, lacI |
| direction | Forward |
| function | Rep |
| ligands | tetracycline |
| negative_regulators | 1 |
| o_h | |
| o_l | |
| positive_regulators | |
| protein | TetR |
| swisspro | P04483 |
| tag | LVA |