Reporter
Part:BBa_K1520014
Designed by: yue wu Group: iGEM14_Nanjing-China (2014-09-22)
Ptet-rbs-rfp-Ter
Ptet-rbs-rfp-Ter. It produces rfp when there is less tetR.
From left to right, Marker（DL2000）, Ptet, Ptet-rbs-rfp, Ptet-rbs-rfp-T. The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/negative
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//promoter
//regulation/negative
//rnap/prokaryote/ecoli/sigma70
| biology | |
| control | aTc, tetracyline |
| direction | Forward |
| negative_regulators | 1 |
| o_h | |
| o_l | |
| positive_regulators |