Composite

Part:BBa_K1520009

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-22)

Plac-rbs-luxI-Ter

Plac-rbs-luxI-Ter.

It produces luxI when there is less lacI or most of lacI is occupied by IPTG.

K1520009.jpg

From left to right, Marker(DL2000), Plac, Plac-rbs-luxI, Plac-rbs-luxI-T. The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 863
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/biosynthesis/ahl
//function/cellsignalling
Parameters
biology
controlIPTG, lacI
directionForward
ec_numnone
keggnone
negative_regulators1
o_h
o_l
positive_regulators
proteinluxI-LVA
swissproP12747
tagLVA
uniprotP48445