Composite

Part:BBa_K1520004

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-22)

Prlux-rbs-lacI-Ter

Prlux-rbs-lacI-Ter. It worked out an expression of lacI in the presence of luxR and AHL.

K1520004.jpg

From left to right M：Marker（DL2000）, a: Prlux, Prlux-rbs-lacI, Prlux-rbs-lacI-T. The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
Parameters
controlluxR, HSL
directionForward
functionRep
ligandsallolactose, IPTG
negative_regulators
o_h
o_l
positive_regulators1
proteinLacI
swissproP03023
tagLVA