Coding

Part:BBa_K1510931

Designed by: Wang,Ching-Yun   Group: iGEM14_NYMU-Taipei   (2014-10-10)


Sequence integrated the luxR gene and pluxR promoter together.

We suppose to conduct the quorum sensing AHL-LuxR system in probiotics and S.mutans, thus we connect the luxR coding gene and the pluxR promoter which aims to be activated by the AHL-LuxR complex.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1019


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Categories
Parameters
None