Coding

Part:BBa_K1510929

Designed by: Wang,Ching-Yun   Group: iGEM14_NYMU-Taipei   (2014-10-09)


Circuit injected in Streptococcus mutans that allows S.m. yield N-Acyl homoserine lactone.

This circuit mainly contains luxI gene which stands for AHL synthetase coding sequence. AHL is the communicator signal we adopt to connect Streptococcus mutans and Escherichia coli with S.m. killing gene. While the chemical signal AHL is successfully generated, the killing gene(Endolysin coding sequence) inside E.coli would be triggered and begins to destruct the cell wall of our target harmful germ, Streptococcus mutans.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 637
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None