Part:BBa_K1510820

RFP indicated INPNC performance evaluation

We anneal a RFP at the end of our INPNC display gene.We adopted a new method that measuring fluorescence light and O.D value of our modified e-coli to test the efficiency of INPNC expression. We successfully derived INPNC expression quantity and expression curve though time. And successfully proved that our targeted gene CSP16 is on the surface of our modified e-coli.

First test After centrifugation, the supernatant is clear; we could indicate that CSP16 is not secreted out of cell. Also, indicated from the red fluorescence of the pellet, we could say that INPNC protein display CSP16 and RFP on the surface.

Second test At the beginning, the population of E. coli is small as well as OD, so initial figure (Fluorescence /OD) is large. As time proceeds, Fluorescence /OD level increases dramatically, indicating that the INPNC protein is being greatly expressed. The OD level reaches a plateau at the end.

Fluorescence level /OD level indicates the display function of INPNC through growth time.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1036
  Illegal AgeI site found at 2220
  Illegal AgeI site found at 2332
  
• 1000
  COMPATIBLE WITH RFC[1000]