Coding

Part:BBa_K1510011

Designed by: Chien-Yung Huang   Group: iGEM14_NYMU-Taipei   (2014-10-06)

Extracellular endolysin

By putting strong constitutive promoter, J23100 in front of yebF and its ribosomal biding site. The recombinant endolysin should be secreted. By adding this part, we are able to have recombinant endolysin form Phage M!02 outside E.coli. In our circuit, we use this circuit to see if endolysing from specific phage can be expressed by E.coli and successfully kill Strepptococcus Mutans. This is an extension of 2013 Arizona Team, BBa_K1190003, by varying the product being carried extracellularlly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None