Composite

Part:BBa_K1510007

Designed by: Wei-Tai Chen   Group: iGEM14_NYMU-Taipei   (2014-10-06)


sRNA targets G protein mRNA in S.mutans

veg promoter(constitutive promoter in S.mutans+ G protein sRNA+ MicC scaffold+ terminator. Constitutive promoter(veg promoter) is came from Bacillus subtilis, and MicC scaffold is came from E.coli k-12 MG1655.Other parts are synthesized by primer. This part will transcibe into sRNA, and sRNA will bind to mRNA of SGP(S.mutans G protein), prevent SGP mRNA from translating. Because G protein is a biofilm formation-related protein, this part is created to decrease biofilm formation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None