Carotenoid Cleavage Dioxygenase 1 (CCD1)

Encodes a protein with 9-cis-epoxycarotenoid dioxygenase activity. The enzyme was shown to act on a variety of carotenoid including β-carotene, lutein, zeaxanthin, and all-trans-violaxanthin. When those compounds are used as substrates, the major reaction product detected is a C14 dialdehyde: 4,9-dimethyldodeca-2,4,6,8,10-pentaene-1,12-dial. The enzyme did not cleave as efficiently carotenoids containing 9-cis-double or allenic bonds.

Testing Result :

Function of Rose Fragrance Generator

Method:

Submit beta-ionone standard and extract of our rose fragrance generator to Gas Chromatography (GC) respectively

Result:

Beta-ionone standard

We injected the beta-ionone standard, extracted by ethyl ehanol, into Gas chromotagraphy which existed an explicit curve at 27.5 minute.

Extract of rose fragrance generator

Then, we injected the sample which had been extracted by Ethyl ethanol also existed curves at around 27 minutes. However, the peak quality is not as good as standard.

Conclusion:

Success in generating rose fragrance beta-ionone from our genetic engineered E.coli

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1071
Illegal XhoI site found at 88
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1521
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 602

Part:BBa_K1507002