Regulatory

Part:BBa_K1500999

Designed by: Kevin Yang   Group: iGEM14_UChicago   (2014-10-17)

ParoF promoter

Used in BBa_1500001, this promoter drives expression of the AroF promoter in wild-type E. coli. Here, it is used as a tyrosine-repressible promoter sensitive to the transcription factor TyrR. The 200 base pairs directly upstream of the AroF gene are cloned here, shown to be functional in Keasling et al 2014 (http://www.nature.com/ncomms/2013/131017/ncomms3595/abs/ncomms3595.html?message-global=remove).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None