Coding

Part:BBa_K1500998

Designed by: Kevin Yang   Group: iGEM14_UChicago   (2014-10-17)


MutD

It is unmutated and still requires the MutD51 (L73W) mutation to become functional in its mutagenic dominant negative form. MutD (dnaQ) gene product controls the editing capacity of pol III (a proofreading DNA exonuclease) and mutD5 mutation leads to a reduction in mismatch repair.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 46
    Illegal AgeI site found at 535
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None