Coding

Part:BBa_K1500997

Designed by: Kevin Yang   Group: iGEM14_uchicago_IGSB   (2014-10-17)


MutL

Along with MutS and MutH, initiates repair cascade by recognizing base mismatches/indels of up to 4 bp (dam-directed mismatch repair). This is the wild-type version; it requires the mutation E32K to become active.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 87
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 87
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 87
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 87
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None