Generator

Part:BBa_K150003

Designed by: Maximilian Hoerner, Christian Moritz, Anna Stoeckl, Yin Cai, Stephen Kraemer   Group: iGEM08_Heidelberg   (2008-10-21)

Chloramphenicol resistance cassette

Chloramphenicol resistance cassette including promotor, RBS, coding sequence and terminator.
In a short characterization it was shown that this part leads to acceptable resistance up to 100 µg/ml chloramphenicol, even in 500 µg/ml unmistakably cell growth can be detected. The effect of the solvent ethanol can even be at high concentrations neglected.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None