Translational_Unit

Part:BBa_K1500013

Designed by: Kevin Yang   Group: iGEM14_UChicago   (2014-10-17)


ParoF--mCherry--dam

Dam is involved in methylating DNA, overexpression causes 6.4 * 10^-4 /// 6.4 * 10^4 mutation rate increase, mostly catalyzing transversions. It requires no mutations and simple overexpression is sufficient to cause its mutagenic activity. In this plasmid, it is placed downstream of the tyrosine-repressible promoter ParoF and reporter gene mCherry to fulfill our goals to improving an established system of directed evolution.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1283
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None