Translational_Unit

Part:BBa_K1500012

Designed by: Rivka Baker Keusch   Group: iGEM14_UChicago   (2014-10-17)


ParoF--mCherry--EmrR

A plasmid that contains the tyrosine-repressible promoter ParoF, followed by an RBS (RBS.3, medium strength), mCherry-LVA as a reporter gene directly downstream, followed by an RBS and the mutator EmrR. The plasmid is an actuator module of our project which contains an optimized promoter, a reporter, and a mutators. The transcription factor TyrR can bind to ParoF and repress it when itself bound to tyrosine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1407
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None