Translational_Unit

Part:BBa_K1500008

Designed by: Kevin Yang   Group: iGEM14_UChicago   (2014-10-17)


IPTG-GFP mutated MutY

The MutY gene cloned into the IPTG-GFP plasmid for characterization. It is involved in G-A mismatch DNA repair. Favors GC to TA Transversions when impaired. This is the dominant negative mutagenic version of the gene that contains a mutation from valine to alanine at the 45th residue.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


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Categories
Parameters
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