Translational_Unit

Part:BBa_K1500007

Designed by: Ryan Beckner   Group: iGEM14_UChicago   (2014-10-17)


IPTG->GFP+mutH(E56A)

This part contains GFP and the mutH(E56A) protein under the control of an IPTG-inducible promoter. MutH(E56A) has been shown to have mutagenic activity E. coli when expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1342
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


[edit]
Categories
Parameters
None