Translational_Unit

Part:BBa_K1500001

Designed by: Kevin Yang   Group: iGEM14_UChicago   (2014-10-17)


ParoF-mCherry backbone

A plasmid that contains the tyrosine-repressible promoter ParoF, followed by an RBS (RBS.3, medium strength) and mCherry-LVA as a reporter gene directly downstream. We used it to insert our combinations of mutator genes directly downstream of the ParoF promoter, forming the actuator module of our project which contains an optimized promoter, a reporter, and one or more mutators. The transcription factor TyrR can bind to ParoF and repress it when itself bound to tyrosine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None