Signalling

Part:BBa_K150000

Designed by: Pascal Kraemer, Marika Ziesack, Kathrin Nussbaum and Andreas Kuehne   Group: iGEM08_Heidelberg   (2008-10-21)

3OC6HSL Sender Controlled by constitutive medium strong Promoter

This part generates the LuxI enzyme which produces AHL (3OC6HSL). It is created with a moderate strong constitutive promoter (BBa_J23107) and a 3OC6HSL (AHL) sender device (BBa_F1610).

Usage and Biology

AHL, a cell-cell signaling molecule is produced by the LuxI protein. The LuxI protein is expressed due to the luxI gene which is under the control of a constitutive promoter (BBa_J23107). For this reason no input is needed for induction of gene expression. As output AHL can be measuredJ23107]). For this reason no input is needed for induction of gene expression. As output AHL can be measured (see experience for a quantitve AHL production determination).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 698
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None