Coding

Part:BBa_K1499501

Designed by: Jovita Byemerwa   Group: iGEM14_StanfordBrownSpelman   (2014-09-29)

Endo-1,4-beta-glucanase (cellulase gene)

Endo-1,4-&-beta-glucanase (EG 1) is an enzyme that breaks down the 1,4-beta-D-glycosidic linkages in cellulose to produce glucose. The coding sequence of this gene was from Neisseria Sicca bacterium that uses cellulose acetate as its sole carbon source. This enzyme is specific for 1,4-beta linkages in cellulose. The molecular mass of EG 1 is 50kDa and its isoelectric point is 5.0. EG 1 contains a carbohydrate binding module at its N-terminus.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 313
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Moriyoshi K et al. (2003) Role of endo-1,4-beta-glucanases from neisseria sicca SB in synergistic degradation of cellulose acetate. Biosci Biotechnol Biochem. 2003 Feb;67(2):250-7/ Link: www.ncbi.nlm.nih.gov/pubmed/12728982

2. Sakai K et al. (1996) Biodegradation of cellulose acetate by Neisseria sicca. Bioscience, Biotechnology, Biochemistry 10: 1617-1622. PMID: 8987659.

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Categories
Parameters
n/aEndo-1,4-beta-glucanase (cellulase gene)