Part:BBa_K1499204

MntH with amber stop codon

This part encodes the manganese transporter, MntH, with one UAG stop codon in place of a leucine codon. MntH is involved with manganese homeostasis, a critical process for dealing with free radicals generated upon exposure to radiation that could potentially cause DNA damage.

Usage and Biology

Deinococcus radiodurans is a highly radiation-resistant bacterium that can survive after up to 12,000 Gy (absorbed radiation dose, Gray). The unique genes that allow it to better handle radiation exposure may provide ways to make E. coli and other organisms more resistant for applications of synthetic biology in tough environmental conditions, like space. Manganese is a cofactor for many of the radiation stress mechanisms in the cell, and so manganese transporters bring manganese into the cell during radiation stress. These transporters are particularly well-known in D. radiodurans, and expressing these proteins in E. coli has shown promise [1].

This part, as a part of the [http://2014.igem.org/Team:StanfordBrownSpelman/Amberless_Hell_Cell Amberless toolkit], should be used in Amberless E. coli. As it has one TAG stop, it will not express in other organisms. It will work in conjunction with the expression of the BBa_K1499251 supP tRNA part and a promoter/RBS in amberless cells

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 135
  Illegal NgoMIV site found at 927
  Illegal NgoMIV site found at 1000
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Haiyan, S & Baoming, T (2010). Radioresistance analysis of Deinococcus radiodurans gene DR1709 in Escherichia coli.African Journal of Microbiol. Research, 4(13): 1412-1418.