Part:BBa_K1491036

Constitutive Dead ER Intein T7 RNAP with YFPH

T7 RNA polymerase that contains the dead version estrogen receptor intein with a constitutive promoter. YFP is added to the end of the plasmid so that the amount of T7 RNA polymerase that is produced can be quantified through fluorescence analysis of YFP. Used as a control.

Characterization

The fluorescence of the sensor and its controls were measure in the red and yellow channels with and without the presence of beta-estradiol over several time points.

*The magnitude of fluorescence of T7 RNA polymerase between the red and yellow channels was so large that they are presented on two different graphs.

TECAN Parameters to Detect RFP Signal from the Sensor:

emission: 585 nm

excitation: 607

bandwidth: 10nm

gain: 175

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2525
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2950
  Illegal AgeI site found at 2914
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2425