Device

Part:BBa_K1491033

Designed by: Danielle Peters   Group: iGEM14_Carnegie_Mellon   (2014-09-22)

T7 promoter-RBS-RFP-T7 Terminator

T7 Promoter with a RBS (BBa_S04423), RFP (BBa_E1010) and a T7 terminator (BBa_B0016). Allows RFP to be transcribed and translated when T7 RNA polymerase is present in the cell.

This plasmid was used in a two plasmid system to detect estrogen in water.

Sensor.png

The sensor final sensor system contains a YFP coding region to report the amount of T7 RNA Polymerase that is produced. The following graphs show the fluorescence of the system and its controls before and after the YFP coding region was added in Top10 cells. Two graphs are shown because of the high magnitude of RFU in the RNAP sample after YFP was added. The following graphs show the amount of fluorescence in the red channel, so RFU is indicative of the amount of RFP that was synthesized in the two plasmid system.

Beforeafteryfp.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 763
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 604
    Illegal AgeI site found at 716
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None