Coding

Part:BBa_K1491024

Designed by: Danielle Peters   Group: iGEM14_Carnegie_Mellon   (2014-09-19)


ER Intein

The intein contains the human E2 estrogen ligand binding domain. When estrogen binds to the receptor, the intein splices out of the protein and joins the N and C terms of the protein, thereby activating it. This allows for an estrogen sensitive protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 983
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1408
    Illegal AgeI site found at 1372
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 883


[edit]
Categories
Parameters
None