Composite

Part:BBa_K1491021

Designed by: Danielle Peters   Group: iGEM14_Carnegie_Mellon   (2014-09-15)

Constitutive Codon Optimized Super Nova

Codon optimized version of the monomeric superoxide generator Super Nova (BBa_K1491017) with a constitutive promoter (BBa_J23100) and a ribosome binding site (BBa_B0034). Super Nova is the monomeric form of Killer Red (BBa_K1184000).

Of the three superoxide generators, Supernova had the biggest decrease in viability following photobleaching when compared to its control. It also had the largest decrease in fluorescence following photobleaching. Fluorescence was measured at (ex/em = 585nm/610nm).

386px-ConstitutiveSuperoxides.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 111
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None