Composite

Part:BBa_K1487066

Designed by: Samantha Bermudez   Group: iGEM14_UT-Dallas   (2014-10-06)


eYFP Reporter for acfD gRNA target with strong promoter.

This part contains a 20bp complementary target sequence for accessory colonization factor D (acfD) that can be targeted by the gRNA from Part:BBa_K1487024. The target sequence is upstream from ribosome binding site Part:BBa_B0034 and a coding region for enhanced yellow fluorescent protein (Part:BBa_E0030). This reporter was placed under a strong constitutive reporter (Part:BBa_J23119).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None