Reporter

Part:BBa_K1487051

Designed by: Samantha Bermudez   Group: iGEM14_UT-Dallas   (2014-10-06)


Target sequence for tcpD + RBS + eYFP

This part contains a 20bp complementary target sequence for toxin coregulated pilus D (tcpD) that can be targeted by the gRNA from Part:BBa_K1487030. The target sequence is upstream from ribosome binding site Part:BBa_B0034 and a coding region for enhanced yellow fluorescent protein (Part:BBa_E0030).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None