Tag

Part:BBa_K1486034

Designed by: EPFL iGem team 2014   Group: iGEM14_EPF_Lausanne   (2014-09-27)

Yeast optimized superfolder GFP C-terminus (215-238)

This part is the C-terminus of a Yeast optimized sfGFP. It contains the 24 last amino-acids, and needs to bind the 214 amino acids long N-terminus fragment to recover fluorescence. Its goal is to provide readout in protein complementation assays, where two proteins tagged with the two termini come in conctact.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/gfp
//chassis/eukaryote/yeast
//direction/forward
//function/reporter/fluorescence
Parameters
biology
chassisSaccharomyces Cerevisiae
colorGreen
directionForward
emission510
excitation480
functionReporter