Part:BBa_K1486031

CaUra3 selection marker

This part is a selection marker for yeast and allows yeasts deficient in the Ura3 gene to grow in media lacking uracil. It contains the gene Ura3 of Candida albicans, a TEF promoter and a TEF terminator. We fused it to our constructs to select the transformants after homologous recombinations.

Source

It was extracted by PCR from plasmids we ordered to addgene: Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3.

Reference

Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae. Lee et al (PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902. Print 2013. PubMed)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 477
  Illegal PstI site found at 166
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 477
  Illegal PstI site found at 166
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 477
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 477
  Illegal PstI site found at 166
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 477
  Illegal PstI site found at 166
  
• 1000
  COMPATIBLE WITH RFC[1000]