R.reniformis luciferase + ADH1 terminator + Kanamycin resistance
This part is a tag Renilla renifomis luciferase (rLuc) associated with a 13 amino acids linker, along a terminator and kanamycin resistance. We used it as a control of the expression and activity of luciferase in the budding yeast S.cerevisiae, by targeting the gene Pbs2. The luciferase is the one from the organism Renilla reniformis, needs the substrate coelenterazine and is ATP independent. Coelenterazine has a spontaneous hydrolysis reaction, and so emits light even without luciferase.
Sequence and Features
- 10Illegal PstI site found at 1490
- 12Illegal PstI site found at 1490
- 21COMPATIBLE WITH RFC
- 23Illegal PstI site found at 1490
- 25Illegal PstI site found at 1490
- 1000COMPATIBLE WITH RFC
Studies of Dynamic Protein-Protein Interactions in Bacteria Using Renilla Luciferase Complementation Are Undermined by Nonspecific Enzyme Inhibition: Stavroula K. Hatzios, Simon Ringgaard, Brigid M. Davis, Matthew K. Waldor.
The coding sequence of this luciferase was generously given by Dr. Matthew Waldor's laboratory.
|resistance||Kanamycin & chloramphenicol (backbone)|